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PrimerPlex designs specific capture probes for direct hybridization assays and primer pairs for Allele Specific PCR Extension (ASPE) assays. The assays are used for high throughput SNP applications such as genotyping, pathogen detection, strain typing, and haplotyping.
What's New
Version 2.70:
- The upgrade accommodates the latest genomic databases available at the NCBI BLAST server and fixes to reported problems.
- Now supports the MagPlex TAGs in addition to the Microplex xTAGs. Use of MagPlex TAGs eliminates the separate step of coupling content specific probes to the MagPlex microspheres. The TAG addition is instrument specific. A user can choose an instrument based on the number of sequences being multiplexed. PrimerPlex will make instrument specific TAGs available for tagging. The TAGs are automatically appended with the ASPE primers, checked for their uniqueness and their secondary structure formations, to avoid minimal cross reactivity with the oligo and assuring success in a multiplex reaction. Users can override the program's recommendations and append the TAGs as per their requirements as well.
- Now supports designing of multiplex primers for standard PCR assays. The algorithms runs through hundreds of primer sets to find primers that not only work efficiently for the sequence but also do not cross hybridize with any other sequence in the pool, or interact with other primer sets of other sequences to assure high signal strength. The design parameters have now been optimized, based on empirical and theoretical evidence, to improve the success in a multiplex reaction. Primers for standard multiplex PCR are designed for different amplicon lengths, different enough to form discernible bands when visualized on a gel. The facility to locate primers in the region of your interest by specifying a search range in the templates chosen for multiplexing has also been included.
Version 2.70:
- The upgrade accommodates the latest genomic databases available at the NCBI BLAST server and fixes to reported problems.
- Now supports the MagPlex TAGs in addition to the Microplex xTAGs. Use of MagPlex TAGs eliminates the separate step of coupling content specific probes to the MagPlex microspheres. The TAG addition is instrument specific. A user can choose an instrument more...
Requirements
Intel/PPC, OS X 10.4.2 or later
Intel/PPC, OS X 10.4.2 or later
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PrimerPlex designs specific capture probes for direct hybridization assays and primer pairs for Allele Specific PCR Extension (ASPE) assays. The assays are used for high throughput SNP applications such as genotyping, pathogen detection, strain typing, and haplotyping.
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Markus Winter reviewed on 19 Sep 2008
But then again, if the price is important to you, then they probably don't want you as a customer anyway
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