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Sequencher free download for Mac


Version 5.4.6

Sequence analysis of DNA.

One-Time Purchase

Sequencher overview

Sequencher is the industry standard software for DNA sequence analysis. It works with all automated sequencers and is widely known for its lightning-fast contig assembly, short learning curve, user-friendly editing tools, and superb technical support. First released almost 15 years ago, Sequencher is currently used for sequence analysis tasks in every major genomic and pharmaceutical company as well as numerous academic and government labs in over 40 countries around the world. Life Science researchers use Sequencher for many diverse DNA sequence analysis applications including de novo gene sequencing, mutation detection, forensic human identification, systematics, and more.

Sequencher capabilities include heterozygote and SNP detection and analysis, cDNA to Genomic DNA large gap alignment, comparative sequencing, support for confidence scores, ORF translation, GenBank feature import, and restriction enzyme mapping. It has many new capabilities that can reduce the time required to identify and validate heterozygotes and SNPs in your sequences.

Pricing for Sequencer must be obtained by requesting a quote, which you can do here.

What’s new in version 5.4.6

Updated on Oct 29 2020

Version 5.4.6:
What’s Changed
  • Email address required for NCBI BLAST searches. In accordance with NCBI guidelines you will now be prompted for an email address the first time you use BLAST from Sequencher Connections.
  • Sequencher Connections Can Now Connect to https://ncbi.nlm.nih.gov/ NCBI has changed their web protocol to the more secure https. We have made changes to ensure that Connections can connect to this new server.
  • Historic Connections Sessions Can Still be Opened. A Connections Session created before the NCBI URL changeover can still be viewed as a historic Session.
  • Change to Historic Session Message in Webview. The message that appears in Webview has been altered and will be displayed when Connections cannot connct to NCBI.
  • Rare Font Issue on Windows 10 Fixed. A rare font issue that only affects Windows 10 users of Sequencher has been fixed. This does not affect older versions of Windows.





315.2 MB

Developer’s website




App requirements

  • Intel 64
  • Mac OS X 10.7 or later
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Jun 6 2008
Jun 6 2008
Version: 4.8
When a TRUE native version for Mactel?
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