EnzymeX

The interface is wonderful and extremely intuitive. It is terrific, both functionally, and visually! Unfortunately, there are a few errors in the program. I emailed the authors, but received a form reply, which is disappointing. Firstly, I cannot trust the recommended buffer combinations! For example, BamHI is listed in NEB 1, 2, 3 and 4 as 75%, 100%, 50% and 75% respectively. From the NEB website, activity is actually 75%, 100%, 100% and 100%. Secondly, ORFs and restriction sites that span across the origin of circular vectors are not identified! Unfortunately, while EnzymeX appears to function as expected otherwise, these two semi-major bugs makes me wary about relying too heavily on this program. From the authors' reply, it appears it is no longer in development. "Unfortunately Papers takes up most of our time these days, so it could take a while before this feature would appear." This is a pity, as these bugs should not be too difficult to fix.

Very nice program for managing DNA sequences. Compact & stylish. Handy new features include virtual digestions, importing directly from BLAST. Some nice features I'd like to see in future versions - abililty to define regions of DNA (gene, promoter, polylinker, etc), circular graphical map.

Better than the reference section of the NEB catalogue in many respects (though lacking the plasmid maps) so a very useful addition to a biochemists/molecular geneticists toolkit. Together with 4Peaks from the same developers these apps are great. Certainly saves you from having to hunt down the catalogue in the lab...