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EnzymeX is a program for molecular biologists, developed to help you determine which restriction enzymes you should use to cut your DNA of interest. It now is a complete DNA sequence analysis and editing program, which contains powerful and unique features and is above all user friendly. Never before was analyzing and editing your DNA constructs so easy and so much fun!

What's New

Version 3.0: Release notes were unavailable when this listing was updated.


Mac OS X 10.3 or later

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EnzymeX User Discussion

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Corkroom Member IconReview+6

The interface is wonderful and extremely intuitive. It is terrific, both functionally, and visually! Unfortunately, there are a few errors in the program. I emailed the authors, but received a form reply, which is disappointing.

Firstly, I cannot trust the recommended buffer combinations! For example, BamHI is listed in NEB 1, 2, 3 and 4 as 75%, 100%, 50% and 75% respectively. From the NEB website, activity is actually 75%, 100%, 100% and 100%.

Secondly, ORFs and restriction sites that span across the origin of circular vectors are not identified!

Unfortunately, while EnzymeX appears to function as expected otherwise, these two semi-major bugs makes me wary about relying too heavily on this program. From the authors' reply, it appears it is no longer in development. "Unfortunately Papers takes up most of our time these days, so it could take a while before this feature would appear." This is a pity, as these bugs should not be too difficult to fix.

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Version 3.1
Rossmcd Member IconReview+0

Very nice program for managing DNA sequences. Compact & stylish. Handy new features include virtual digestions, importing directly from BLAST. Some nice features I'd like to see in future versions - abililty to define regions of DNA (gene, promoter, polylinker, etc), circular graphical map.

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Version 3.1
JKT Member IconReview+44

Better than the reference section of the NEB catalogue in many respects (though lacking the plasmid maps) so a very useful addition to a biochemists/molecular geneticists toolkit. Together with 4Peaks from the same developers these apps are great.

Certainly saves you from having to hunt down the catalogue in the lab...

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Version 2.5
user icon+4
Version 3.1
> 3 4


Current Version (3.x)


Downloads 6,168
Version Downloads 128
License Free
Date 02 May 2006
Platform OS X / PPC 32 / Intel 32
Price Free